Landon Collins, Olivier Vernon and Damon Harrison took a knee during the national anthem on Sunday before the Giants' Week 3 game against the Eagles.
Many others on the team -- including coaches -- stood and locked arms during the anthem.
Giants co-owners John Mara and Steve Tisch offered a joint statement on Saturday in response to President Trump's call for NFL owners to fire players who kneel during the anthem.
"Comments like we heard last night from the president are inappropriate, offensive and divisive. We are proud of our players, the vast majority of whom use their NFL platform to make a positive difference in our society," Mara and Tisch said.