LB B.J. Goodson (ankle), DT Damon Harrison (ankle), OL Justin Pugh (back), LB Kelvin Sheppard (groin) all missed Giants practice on Wednesday.
CB Donte Deayon (ankle), G D.J. Fluker (knee), LB Devon Kennard (quad), LB Calvin Munson (quad), DE Jason Pierre-Paul (knee), and DT Dalvin Tomlinson (ankle) were all limited participants.
Pugh will be out for the team's matchup against the Chiefs this Sunday, per NFLN's Ian Rapoport.
Pugh sustained the injury against the 49ers on Sunday, exited the game and didn't return.
It would be a huge loss to the Giants as Pugh served as a versatile asset that could play anywhere on the line. He has already become the second starter next to C Weston Richburg to miss time this season.
The Giants signed G John Greco on Monday to fill Pugh's void.