The Giants ruled center Weston Richburg and running back Paul Perkins out for Sunday's game against the Los Angeles Chargers.
New York also listed defensive ends Olivier Vernon, Jason Pierre-Paul and Avery Moss, and offensive guard John Jerry as questionable with various injuries.
Pierre-Paul told SNY's Taylor Rooks on Thursday that he'd be ready to go on Sunday.
Richburg has been in the league's concussion protocol, and Brett Jones is likely to be the team's starting center on Sunday, according to Inside Football's Patricia Traina, as the Giants seek their first win of the season.
Perkins has been dealing with a rib injury, meaning Orleans Darkwa and Wayne Gallman Jr. are expected to split the load at running back, according to NJ.com's Dan Duggan.
Pierre-Paul vowed to play on Sunday despite battling a shoulder injury that he sustained in last week's loss to the Tampa Bay Buccaneers.
Vernon has been dealing with an ankle injury, while Jerry and Moss are dealing with hamstring and shoulder injuries, respectively.
Wide receiver Odell Beckham Jr. was not listed on the injury report despite being limited in practice earlier this week due to finger and ankle injuries.